Coding

Part:BBa_K4309000:Design

Designed by: Yanqiu Tian   Group: iGEM22_NPU-CHINA   (2022-09-29)


Laccase（Bacillus）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1207
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1275
    Illegal BsaI.rc site found at 1509
    Illegal SapI.rc site found at 286


Design Notes

The laccase gene derived from Bacillus subtilis was successfully expressed in Escherichia coli and the degradation efficiency was verified. These works are of great significance to our detailed design of the sequence.


Source

From Bacillus.

References